Mouse CBLB(E3 ubiquitin-protein ligase CBLB) ELISA Kit

Mfr.No.

ELK9290

Sensitivity

0.062 ng/mL

Detection Range

0.16-10 ng/mL

Reactivity

Mouse

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

CBLB, Signal transduction protein CBL-B, SH3-binding protein CBL-B, Casitas B-lineage lymphoma proto-oncogene b, RING finger protein 56, CBLB ; Signal transduction protein CBL-B ; SH3-binding protein CBL-B ; Casitas B-lineage lymphoma proto-oncogene b ; RING finger protein 56

Recovery

Matrices listed below were spiked with certain level of recombinant CBLB and the recovery rates were calculated by comparing the measured value to the expected amount of CBLB in samples.

Matrix	Recovery range	Average
serum(n=5)	84-98%	91%
EDTA plasma(n=5)	86-98%	92%
Heparin plasma(n=5)	95-107%	101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CBLB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	83-101%	87-98%	79-92%	87-101%
EDTA plasma(n=5)	89-97%	85-94%	87-98%	92-101%
Heparin plasma(n=5)	83-98%	86-96%	81-97%	85-96%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
10.00	1.952	1.867
5.00	1.755	1.670
2.50	1.211	1.126
1.25	0.797	0.712
0.63	0.538	0.453
0.32	0.335	0.250
0.16	0.232	0.147
0.00	0.085	0.000

Limit Of Detection

10 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CBLB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CBLB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CBLB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CBLB in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	Q3TTA7

Applications	ELISA
Research Area	Enzyme & Kinase;

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SKU	Supplier No.	Size	Your price	Quantity
BHE152011028	ELK9290	48T	$320		Add to Cart
BHE152011028	ELK9290	96T	$458		Add to Cart

Mouse CBLB(E3 ubiquitin-protein ligase CBLB) ELISA Kit

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