Mouse catestatin ELISA Kit

Mouse catestatin ELISA Kit

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Mfr.No.

CSB-E17357m

Alternative Names

catestatin

Reactivity

Mouse

Detection Range

78 pg/mL-5000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-100
EDTA plasma (n=4)	95	91-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C-Peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-99
1:2	Average %	90
	Range %	85-96
1:4	Average %	95
	Range %	87-106
1:8	Average %	95
	Range %	89-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
2.86	0.055	0.059	0.057
5.71	0.147	0.154	0.151
11.43	0.367	0.372	0.370
28.57	0.846	0.855	0.851
50	1.618	1.607	1.613

Species

Mus musculus

Sensitivity

19.5 pg/mL

Limit Of Detection

450 nm

Antigen	catestatin

Applications

ELISA

Research Area

catestatin

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	CatestatinDecreases macrophage function in two mouse models of experimental colitis Rabbi MF et al,Biochem Pharmacol.,2014 Mar 14.pii: S0006-2952(14)00161-0.

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.