Mouse Cartilage oligomeric matrix protein(COMP) ELISA kit

Mouse Cartilage oligomeric matrix protein(COMP) ELISA kit

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Mfr.No.

CSB-EL005778MO

Sensitivity

1.56 ng/mL

Detection Range

6.25 ng/mL-400 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

cartilage oligomeric matrix protein, EDM1, EPD1, MED, MGC131819, MGC149768, PSACH, THBS5, cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple), cartilage oligomeric matrix protein(pseudoachondropl

Recovery

The recovery of human catestatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-101
EDTA plasma (n=4)	94	91-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human catestatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	101
	Range %	97-105
1:2	Average %	83
	Range %	80-87
1:4	Average %	92
	Range %	87-94
1:8	Average %	91
	Range %	87-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.564	2.438	2.501	2.317
10	2.004	2.131	2.068	1.884
5	1.533	1.568	1.551	1.367
2.5	0.905	0.935	0.920	0.736
1.25	0.618	0.608	0.613	0.429
0.625	0.432	0.445	0.439	0.255
0.312	0.297	0.287	0.292	0.108
0	0.183	0.185	0.184

Species

Mus musculus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9R0G6
Tissue Specificity	Expressed only in cartilage, including nasal, knee epiphyseal and rib tissues.

Applications	ELISA
Research Area	COMP

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505313	CSB-EL005778MO	96T	$695	Add to Cart
BHE10505313	CSB-EL005778MO	5x96T	$3336	Add to Cart
BHE10505313	CSB-EL005778MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.