Mouse cAMP response element binding protein(CREB)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E15860m

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

cAMP response element binding protein(CREB)

Recovery

The recovery of human pepsinogen I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	97	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human pepsinogen I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	106
	Range %	100-115
1:4	Average %	97
	Range %	94-112
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q01147

Applications	ELISA
Research Area	CREB

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Oxcarbazepine And Fluoxetine Protects Against Mouse Models Of Obsessive Compulsive Disorder Through Modulation Of Cortical Serotonin And Creb Pathway Arora T et al,Behav Brain Res.,2013 Jun 15;247:146-52.

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