Mouse Calcium-dependent phospholipase A2(PLA2G5) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL018103MO

Sensitivity

15.6 pg/ml

Detection Range

62.5 pg/ml-4000 pg/ml

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Recovery

The recovery of rat PON1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat PON1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	91
	Range %	86-95
1:400	Average %	102
	Range %	97-107
1:800	Average %	91
	Range %	85-97
1:1600	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

UniProt ID	P97391

Applications	ELISA
Research Area	PLA2G5

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10505284	CSB-EL018103MO	96T	$695	Add to Cart
BHE10505284	CSB-EL018103MO	5x96T	$3336	Add to Cart
BHE10505284	CSB-EL018103MO	10x96T	$6602.50	Add to Cart