Mouse CA4/Carbonic anhydrase 4 ELISA Kit PicoKine®

Mfr.No.

EK1940

Sensitivity

<50 pg/ml

Detection Range

78 pg/ml - 5,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.051	0.140	0.238	0.397	0.709	1.235	1.548	2.076

Description

Mouse CA4 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ca4 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Carbonic anhydrase 4; Carbonate dehydratase IV; Carbonic anhydrase IV; CA-IV; Ca4; Car4

Immunogen

Expression system for standard: NS0; Immunogen sequence: E18-S277

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CA4

Subcellular Localization

Cell membrane, GPI-anchor

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	135	612	2184	140	618	2258
Standard deviation	7.02	38.56	104.83	6.86	45.73	119.67
CV (%)	5.2%	6.3%	4.8%	4.9%	7.4%	5.3%

Protein Function

Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4.

Background

Carbonic anhydrase 4 is an enzyme that in humans is encoded by the CA4 gene. It is mapped to 11; 11 C. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IV is a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and of proximal renal tubules. Its exact function is not known, however, it may have a role in inherited renal abnormalities of bicarbonate transport.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	135	147	139	153	143	6.98	4.8%
Sample 2	612	690	628	619	637	30.97	4.8%
Sample 3	2184	2286	2132	2361	2240	88.81	3.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	12351
Gene Name	Ca4
UniProt ID	Q64444

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001467	EK1940	96 wells/kit	$750		Add to Cart

Mouse CA4/Carbonic anhydrase 4 ELISA Kit PicoKine®

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