Mouse CA16-IgM (Coxsackievirus A16-Immunoglobulin M) ELISA kit

Mfr.No.

EM2181

Description

This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with CA16-IgM bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of CA16-IgM in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Alternative Names

CA16-IgM ELISA Kit, Coxsackievirus A16-Immunoglobulin M ELISA Kit

Reactivity

Mouse

Detection Range

Qualitative

Sample Type

Cell lysates, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Qualitative

Assay Duration

3 hours

Sensitivity

Qualitative

Assay Procedure Summary

Step 1:
Wash the plate twice before adding the standard and sample.

Step 2:
Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37°C.

Washing:
Wash the plate three times and immerse for 1min each time.

Step 3:
Add 100ul HRP-antibody working solution, seal the plate and statically incubate for 30 minutes at 37°C.

Washing:
Wash the plate five times and immerse for 1min each time.

Step 4:
Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)

Step 5:
Add 50ul stop solution. Read at 450nm immediately and calculate.

Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5)	37°C for 1 month	2-8°C for 6 months
Average(%)	80	95-100

Notes

Specifically binds with CA16-IgM , no obvious cross reaction with other analogues.

Usage Notes	Microplate reader (wavelength: 450nm) 37°C incubator (CO2 incubator for cell culture is not recommenced.) Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose) Precision single (0.5-10uL, 5-50uL, 20-200uL, 200-1000uL) and multi-channel pipette with disposable tips(Calibration is required before use.) Sterile tubes and Eppendorf tubes with disposable tips Absorbent paper and loading slot Deionized or distilled water

Storage	2-8°C(Sealed), Don't cryopreserve.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10810092	EM2181	48T	$280		Add to Cart
BHE10810092	EM2181	96T	$400		Add to Cart

Mouse CA16-IgM (Coxsackievirus A16-Immunoglobulin M) ELISA kit

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