Mouse C5a(Complement Component 5a) ELISA Kit

Mfr.No.

EM0885

Sensitivity

< 9.375pg/ml

Detection Range

15.625-1000pg/ml

Reactivity

Mouse

Assay Type

Sandwich

Alternative Names

C5a ELISA Kit

Detection Method

Double Antibody

Recovery

Matrices listed below were spiked with certain level of C5a and the recovery rates were calculated by comparing the measured value to the expected amount of C5a in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	85-104	97
EDTA plasma(n=5)	88-98	91
heparin plasma(n=5)	85-103	90

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C5a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	92-102%	86-102%	86-102%
EDTA plasma(n=5)	87-98%	84-97%	86-99%
heparin plasma(n=5)	80-93%	86-96%	87-100%

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Precision

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Background

C5a

Notes

For Research Use Only

Applications	ELISA
Applications Notes	C5a ELISA Kit allows for the in vitro quantitative determination of C5a concentrations in serum, plasma, tissue homogenates and other biological fluids.

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10803751	EM0885	96T	$400		Add to Cart

Mouse C5a (Complement Component 5a) ELISA Kit

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