Mouse C-X-C motif chemokine 13 (Cxcl13/Blc/Scyb13) ELISA kit

Mouse C-X-C motif chemokine 13 (Cxcl13/Blc/Scyb13) ELISA kit

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Mfr.No.

CSB-E16832m

Alternative Names

C-X-C motif chemokine 13 (Cxcl13/Blc/Scyb13), ANGIE, ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13, B-cell chemoattractant, B-cell-attracting chemokine 1, B-cell-homing chemokine (ligand for Burkitt s lymphoma receptor-1), B-lymphocyte chemoattractant, c

Reactivity

Mouse

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Serum, Cell culture supernatants, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-106
EDTA plasma (n=4)	88	82-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	84-96
1:2	Average %	94
	Range %	89-98
1:4	Average %	95
	Range %	91-99
1:8	Average %	92
	Range %	89-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
120	2.204	2.103	2.154	2.013
60	1.555	1.454	1.505	1.364
30	0.883	0.862	0.873	0.732
15	0.495	0.506	0.501	0.360
7.5	0.327	0.316	0.322	0.181
3.75	0.234	0.245	0.240	0.099
1.87	0.179	0.175	0.177	0.036
0	0.141	0.140	0.141	?

Species

Mus musculus

Sensitivity

0.096 ng/mL

Limit Of Detection

450 nm

UniProt ID	O55038
Antigen	C-X-C motif chemokine 13 (Cxcl13/Blc/Scyb13)

Applications

ELISA

Research Area

CXCL13

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505528	CSB-E16832m	96T	$495	Add to Cart
BHE10505528	CSB-E16832m	5x96T	$2376	Add to Cart
BHE10505528	CSB-E16832m	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.