Mouse C-C Motif Chemokine 25

Mfr.No.

E1917Mo

Alternative Names

C-C motif chemokine 25;CCL 25;CCL25;Chemokine TECK;Small-inducible cytokine A25;Thymus-expressed chemokine

Reactivity

Mouse

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

16733

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

6.77ng/L

Background

Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

Gene ID	20300
UniProt ID	O35903
Antigen	CCL25

Research Area

Immunology

Protocol

These standard curves of E1917Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	1.699	1.738	1.689
	1.778
800ng/L	0.93	0.913	0.864
	0.897
400ng/L	0.502	0.524	0.475
	0.546
200ng/L	0.186	0.211	0.161
	0.236
100ng/L	0.117	0.132	0.082
	0.146
0ng/L	0.042	0.049	0
	0.056

Precision

We measured random samples of E1917Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	891	45.18	5.1
Intra-Assay	2	18	1423.3	42.52	3
Intra-Assay	3	18	607.8	37.25	6.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109729	E1917Mo	48T	$320		Add to Cart
BHE12109729	E1917Mo	96T	$458		Add to Cart

Mouse C-C Motif Chemokine 25 - CCL25 ELISA Kit

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