Mouse C-C motif chemokine 25(CCL25) ELISA kit

Mouse C-C motif chemokine 25(CCL25) ELISA kit

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Mfr.No.

CSB-EL004789MO

Alternative Names

C-C motif chemokine 25(CCL25), Ckb15, MGC150327, SCYA25, TECK, Ck beta-15, TECKvar, chemokine TECK, small inducible cytokine A25, small inducible cytokine subfamily A (Cys-Cys), member 25, thymus expressed chemokine

Reactivity

Mouse

Detection Range

78 pg/ml - 5000 pg/ml

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse PADI4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	88-105
EDTA plasma (n=4)	96	89-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PADI4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-99
1:2	Average %	95
	Range %	89-101
1:4	Average %	96
	Range %	92-110
1:8	Average %	93
	Range %	86-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
300	2.228	2.230	2.229	2.051
150	1.457	1.378	1.418	1.240
75	0.854	0.822	0.838	0.660
37.5	0.483	0.513	0.498	0.320
18.75	0.355	0.363	0.359	0.181
9.4	0.274	0.259	0.267	0.089
4.7	0.233	0.226	0.230	0.052
0	0.175	0.181	0.178

Species

Mus musculus

Sensitivity

19.5 pg/ml

Limit Of Detection

450 nm

UniProt ID	O35903
Antigen	C-C motif chemokine 25(CCL25)

Applications

ELISA

Research Area

CCL25

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505334	CSB-EL004789MO	96T	$695	Add to Cart
BHE10505334	CSB-EL004789MO	5x96T	$3336	Add to Cart
BHE10505334	CSB-EL004789MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.