Mouse C-C chemokine receptor type 2(CCR2) ELISA kit

Mouse C-C chemokine receptor type 2(CCR2) ELISA kit

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Mfr.No.

CSB-EL004841MO

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

chemokine (C-C motif) receptor 2, CC-CKR-2, CCR2A, CCR2B, CD192, CKR2, CKR2A, CKR2B, CMKBR2, MCP-1-R, MCP-1 receptor, chemokine (C-C) receptor 2, monocyte chemoattractant protein 1 receptor, monocyte chemotactic protein 1 receptor

Recovery

The recovery of mouse PAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	97	95-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-98
1:2	Average %	97
	Range %	92-107
1:4	Average %	93
	Range %	87-98
1:8	Average %	87
	Range %	82-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.576	2.652	2.614	2.460
25	2.044	2.076	2.060	1.906
12.5	1.443	1.504	1.474	1.320
6.25	0.696	0.750	0.723	0.569
3.12	0.466	0.504	0.485	0.331
1.56	0.354	0.369	0.362	0.208
0.78	0.241	0.259	0.250	0.096
0	0.153	0.155	0.154	?

Species

Mus musculus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P51683
Tissue Specificity	Detected in monocyte/macrophage cell lines, but not in nonhematopoietic cell lines.

Applications	ELISA
Research Area	CCR2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505333	CSB-EL004841MO	96T	$695	Add to Cart
BHE10505333	CSB-EL004841MO	5x96T	$3336	Add to Cart
BHE10505333	CSB-EL004841MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.