Matrices listed below were spiked with certain level of Btla and the recovery rates were calculated by comparing the measured value to the expected amount of Btla in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
95
EDTA plasma(n=5)
85-105
93
heparin plasma(n=5)
87-102
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Btla and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-105%
87-101%
86-101%
EDTA plasma(n=5)
88-101%
85-101%
86-100%
heparin plasma(n=5)
85-97%
88-100%
85-98%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q7TSA3
Applications
ELISA
Applications Notes
Btla ELISA Kit allows for the in vitro quantitative determination of Btla concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Btla (B- and T-lymphocyte attenuator) ELISA Kit