Mouse brain natriuretic peptide,BNP ELISA Kit

Mfr.No.

CSB-E07971m

Sensitivity

1.56 pg/mL

Detection Range

6.25 pg/mL-400 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

natriuretic peptide precursor B, BNP, brain type natriuretic peptide, natriuretic protein

Recovery

The recovery of mouse apo-A5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-94
EDTA plasma (n=4)	108	103-112

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse apo-A5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	92
	Range %	86-95
1:2000	Average %	97
	Range %	92-103
1:4000	Average %	93
	Range %	88-98
1:8000	Average %	89
	Range %	84-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P40753

Applications	ELISA
Research Area	NPPB

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
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