Mouse Bone morphogenetic protein 2,BMP-2 ELISA Kit

Mfr.No.

CSB-E04509m

Alternative Names

bone morphogenetic protein 2, BMP2A

Reactivity

Mouse

Detection Range

1.56 pg/mL-100 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat anti-TPO antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-99
EDTA plasma (n=4)	95	90-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat anti-TPO antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	86-101
1:2	Average %	88
	Range %	83-98
1:4	Average %	95
	Range %	90-100
1:8	Average %	95
	Range %	89-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

0.39 pg/mL

Limit Of Detection

450 nm

UniProt ID	P21274
Antigen	Bone Morphogenetic Protein 2

Applications

ELISA

Research Area

BMP2

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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