Mouse Bone marrow proteoglycan(PRG2) ELISA kit

Mouse Bone marrow proteoglycan(PRG2) ELISA kit

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Mfr.No.

CSB-EL018670MO

Sensitivity

0.98 ng/mL

Detection Range

3.9 ng/mL-250 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein), BMPG, MBP, MBP1, MGC14537, bone-marrow proteoglycan, eosinophil granule major basic protein, eosinophil major basic protein, natural killer cell activator, proteoglycan 2, proteoglycan 2 preproprotein

Recovery

The recovery of human IL1F8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	80-92
EDTA plasma (n=4)	100	96-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-92
1:2	Average %	96
	Range %	90-102
1:4	Average %	89
	Range %	85-94
1:8	Average %	88
	Range %	84-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.537	2.598	2.568	2.404
500	2.062	2.091	2.077	1.913
250	1.399	1.368	1.384	1.220
125	0.799	0.771	0.785	0.621
62.5	0.555	0.534	0.545	0.381
31.2	0.374	0.395	0.385	0.221
15.6	0.271	0.294	0.283	0.119
0	0.163	0.165	0.164	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q61878

Applications	ELISA
Research Area	PRG2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505246	CSB-EL018670MO	96T	$695	Add to Cart
BHE10505246	CSB-EL018670MO	5x96T	$3336	Add to Cart
BHE10505246	CSB-EL018670MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.