Mouse BMP-binding endothelial regulator protein(BMPER) ELISA kit

Mouse BMP-binding endothelial regulator protein(BMPER) ELISA kit

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Mfr.No.

CSB-EL002747MO

Alternative Names

BMP binding endothelial regulator, CRIM3, CV-2, CV2, BMP-binding endothelial regulator precursor protein, OTTHUMP00000158859, bone morphogenetic protein-binding endothelial cell precursor-derived regulator, crossveinless 2, crossveinless

Reactivity

Mouse

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human PDPN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	105	103-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PDPN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	91
	Range %	86-95
1:10	Average %	95
	Range %	90-100
1:20	Average %	104
	Range %	99-108
1:40	Average %	88
	Range %	85-91

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.654	2.795	2.725	2.537
800	2.022	2.200	2.111	1.923
400	1.278	1.369	1.324	1.136
200	0.801	0.867	0.834	0.646
100	0.486	0.492	0.489	0.301
50	0.378	0.363	0.371	0.183
25	0.263	0.282	0.273	0.085
0	0.186	0.189	0.188

Species

Mus musculus

Sensitivity

7.81 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q8CJ69
Antigen	BMP Binding Endothelial Regulator

Applications

ELISA

Research Area

BMPER

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505245	CSB-EL002747MO	96T	$695	Add to Cart
BHE10505245	CSB-EL002747MO	5x96T	$3336	Add to Cart
BHE10505245	CSB-EL002747MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.