Matrices listed below were spiked with certain level of Blzf1 and the recovery rates were calculated by comparing the measured value to the expected amount of Blzf1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-103
97
EDTA plasma(n=5)
87-100
96
heparin plasma(n=5)
87-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Blzf1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-100%
85-100%
96-104%
EDTA plasma(n=5)
85-98%
92-100%
92-101%
heparin plasma(n=5)
87-100%
88-99%
85-98%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
UniProt ID
Q8R2X8
Applications
ELISA
Applications Notes
Blzf1 ELISA Kit allows for the in vitro quantitative determination of Blzf1 concentrations in serum, plasma, tissue homogenates and other biological fluids.