Mouse beta2-microglobulin,BMG/beta2-MG ELISA Kit

Mouse beta2-microglobulin,BMG/beta2-MG ELISA Kit

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Mfr.No.

CSB-E13053m

Sensitivity

3.12 ng/mL

Detection Range

12.5 ng/mL-800 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

beta-2-microglobulin, beta chain of MHC class I molecules, beta-2-microglobin, beta2-microglobulin,BMG/beta2-MG,, beta-2-microglobulin, beta chain of MHC class I molecules, beta-2-microglobin, beta2-microglobulin,BMG/beta2-MG,

Recovery

The recovery of human TTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-100
EDTA plasma (n=4)	98	90-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-104
1:2	Average %	96
	Range %	91-101
1:4	Average %	93
	Range %	87-99
1:8	Average %	92
	Range %	84-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.195	2.095	2.145	2.014
750	1.594	1.494	1.544	1.413
375	0.932	0.892	0.912	0.781
187.5	0.506	0.486	0.496	0.365
94	0.352	0.342	0.347	0.216
47	0.229	0.219	0.224	0.093
23.5	0.168	0.162	0.165	0.034
0	0.131	0.130	0.131	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P01887

Applications	ELISA
Research Area	B2M

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506953	CSB-E13053m	96T	$595	Add to Cart
BHE10506953	CSB-E13053m	5x96T	$2856	Add to Cart
BHE10506953	CSB-E13053m	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.