Mouse Beta-Klotho - KLB ELISA Kit

Mfr.No.

E1880Mo

Sensitivity

9.74ng/L

Detection Range

20-4500ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

BetaKlotho;Beta-klotho;BKL;KLB;Klotho beta-like protein

Molecular Weight

120189

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1880Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	480.7	18.88	3.9
Intra-Assay	2	18	1828.7	49.27	2.7
Intra-Assay	3	18	1155.9	42.08	3.6

Background

Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21.

Protocol

These standard curves of E1880Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.098	2.087	2.042
	2.077
1200ng/L	1	0.974	0.929
	0.948
600ng/L	0.388	0.494	0.449
	0.601
300ng/L	0.225	0.216	0.171
	0.206
150ng/L	0.079	0.115	0.069
	0.15
0ng/L	0.042	0.045	0
	0.048

Gene ID	83379
UniProt ID	Q99N32

Research Area	Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109692	E1880Mo	48T	$320		Add to Cart
BHE12109692	E1880Mo	96T	$458		Add to Cart

Mouse Beta-Klotho - KLB ELISA Kit

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