Mouse beta-catenin,beta-cat ELISA Kit

Mouse beta-catenin,beta-cat ELISA Kit

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Mfr.No.

CSB-E11307m

Alternative Names

catenin (cadherin-associated protein), beta 1, 88kDa, CTNNB, DKFZp686D02253, FLJ25606, FLJ37923, OTTHUMP00000209288, beta-catenin, beta-catenin,beta-cat

Reactivity

Mouse

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of Human NOD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-98
EDTA plasma (n=4)	88	82-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of Human NOD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-95
1:2	Average %	96
	Range %	90-102
1:4	Average %	93
	Range %	87-99
1:8	Average %	97
	Range %	93-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.355	2.417	2.386	2.286
800	1.624	1.667	1.646	1.546
400	0.987	1.075	1.031	0.931
200	0.658	0.668	0.663	0.563
100	0.426	0.426	0.426	0.326
50	0.277	0.268	0.273	0.173
25	0.185	0.186	0.186	0.086
0	0.099	0.101	0.100

Species

Mus musculus

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q02248
Antigen	catenin (cadherin-associated protein), beta 1, 88kDa

Applications

ELISA

Research Area

CTNNB1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506954	CSB-E11307m	96T	$695	Add to Cart
BHE10506954	CSB-E11307m	5x96T	$3336	Add to Cart
BHE10506954	CSB-E11307m	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.