Mouse BCAM/Cd239 ELISA Kit PicoKine®

Mfr.No.

EK1547

Sensitivity

<15 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1,000	2,000
O.D.	0.020	0.084	0.155	0.277	0.494	0.888	1.520	2.123

Description

Mouse BCAM/Cd239 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Bcam in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Basal cell adhesion molecule;B-CAM cell surface glycoprotein;Lutheran antigen;CD239;Bcam;Gplu, Lu;

Molecular Weight

67670 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E26-A541

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse BCAM

Subcellular Localization

Membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	105	288	896	112	303	922
Standard deviation	5.67	20.45	61.82	8.06	19.7	58.9
CV (%)	5.4%	7.1%	6.9%	7.2%	6.5%	6.3%

Protein Function

Laminin alpha-5 receptor. May mediate intracellular signaling (By similarity). .

Background

Basal cell adhesion molecule is a protein that in humans is encoded by the BCAM gene. The BCAM gene encodes a glycoprotein expressed on cell surfaces. It is a member of the immunoglobulin superfamily of receptors and cell adhesion molecules. When expressed on erythrocytes, BCAM constitutes the Lutheran (Lu) and Auberger (Au) blood group antigens. And this protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	105	99	112	96	103	3.4	3.3%
Sample 2	288	265	272	281	277	8.57	3.1%
Sample 3	896	845	826	881	862	30.17	3.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	57278
Gene Name	Bcam
UniProt ID	Q9R069
Protein Name	Basal cell adhesion molecule

Applications	ELISA
Research Area	Angiogenesis,Cardiovascular,Cell Adhesion Proteins,Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Neuroscience,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001069	EK1547	96 wells/kit	$499		Add to Cart

Mouse BCAM/Cd239 ELISA Kit PicoKine®

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