Mouse basic fibroblast growth factor,bFGF ELISA Kit

Mouse basic fibroblast growth factor,bFGF ELISA Kit

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Mfr.No.

CSB-E08001m

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

fibroblast growth factor 2 (basic), BFGF, FGFB, HBGF-2, basic fibroblast growth factor bFGF, fibroblast growth factor 2, heparin-binding growth factor 2, prostatropin

Recovery

The recovery of rat SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-91
EDTA plasma (n=4)	98	95-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	93-99
1:2	Average %	94
	Range %	91-97
1:4	Average %	100
	Range %	97-103
1:8	Average %	102
	Range %	99-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
5000	1.978	1.998	1.988	1.846
2500	1.341	1.398	1.370	1.228
1250	0.813	0.834	0.824	0.682
625	0.476	0.498	0.487	0.345
312.5	0.308	0.328	0.318	0.176
156.25	0.245	0.258	0.252	0.110
78	0.202	0.215	0.209	0.067
0	0.141	0.143	0.142	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P15655

Applications	ELISA
Research Area	FGF2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10505220	CSB-E08001m	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.