Mouse BAFF ELISA Kit PicoKine®

Mfr.No.

EK0664

Sensitivity

<10 pg/ml

Detection Range

46.9 pg/ml - 3,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	46.9	94	188	375	750	1500	3000
O.D.	0.037	0.107	0.157	0.247	0.454	0.764	1.214	1.931

Description

Mouse BAFF ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfsf13b in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor (Ligand) superfamily, member 13b ;Tumor necrosis factor (Ligand) superfamily, member 13b, isoform CRA_d ;Tnfsf13b ;mCG_3400 ;

Molecular Weight

34192 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A127-L309

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse BAFF

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	82	287	1221	76	287	1209
Standard deviation	6.47	15.52	67.15	7.14	16.07	72.54
CV (%)	7.9%	5.3%	5.5%	9.4%	5.6%	6%

Background

B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFLSF13B gene.1,2 BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.3All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors4, although BAFF has been known to be active as either a hetero- or homotrimer.5 The standard used in this kit is recombinant mouse soluble BAFF (A127-L309) with molecular weight 23.2KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	82	85	90	80	84	3.76	4.4%
Sample 2	293	335	318	295	310	17.34	5.5%
Sample 3	1221	1161	1374	1312	1267	81.89	6.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	24099
Gene Name	TNFSF13B
UniProt ID	Q3KP92
Protein Name	Tumor necrosis factor ligand superfamily member 13B

Applications	ELISA
Research Area	Adaptive Immunity,B Cells,Cancer,Cytokines,Growth Factors,Growth Factors/Hormones,Immunology,Innate Immunity,Non-CD,Signal Transduction,TNF Superfamily

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000553	EK0664	96 wells/kit	$499		Add to Cart

Mouse BAFF ELISA Kit PicoKine®

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