Mouse B-cell leukemia/lymphoma 2,Bcl-2 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08855m

Alternative Names

B-cell CLL/lymphoma 2, Bcl-2, B-cell lymphoma protein 2, OTTHUMP00000163680

Reactivity

Mouse

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human TWSG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-99
EDTA plasma (n=4)	84	80-88

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TWSG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-96
1:2	Average %	99
	Range %	92-106
1:4	Average %	89
	Range %	83-94
1:8	Average %	92
	Range %	87-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	P10417
Antigen	B-cell CLL/lymphoma 2

Applications

ELISA

Research Area

BCL2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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