Matrices listed below were spiked with certain level of AT1R and the recovery rates were calculated by comparing the measured value to the expected amount of AT1R in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
91
EDTA plasma(n=5)
87-105
96
heparin plasma(n=5)
88-105
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AT1R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-102%
91-103%
87-103%
EDTA plasma(n=5)
82-101%
83-99%
85-95%
heparin plasma(n=5)
83-94%
80-97%
82-98%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
UniProt ID
Q9WVK0
Applications
ELISA
Applications Notes
AT1R ELISA Kit allows for the in vitro quantitative determination of AT1R concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse AT1R (angiotensin II receptor type 1) ELISA Kit