Matrices listed below were spiked with certain level of AT-III and the recovery rates were calculated by comparing the measured value to the expected amount of AT-III in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
97-102
100
EDTA plasma(n=5)
89-105
99
heparin plasma(n=5)
86-104
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AT-III and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
AT-III ELISA Kit allows for the in vitro quantitative determination of AT-III concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse AT-III(anti-Thrombin III) ELISA Kit