Matrices listed below were spiked with certain level of AQP-4 and the recovery rates were calculated by comparing the measured value to the expected amount of AQP-4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-100
94
EDTA plasma(n=5)
91-104
98
heparin plasma(n=5)
88-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AQP-4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
AQP-4 ELISA Kit allows for the in vitro quantitative determination of AQP-4 concentrations in serum, plasma, tissue homogenates and other biological fluids.