Mouse Apoptosis-associated speck-like protein containing a CARD(PYCARD) ELISA kit

Mouse Apoptosis-associated speck-like protein containing a CARD(PYCARD) ELISA kit

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Mfr.No.

CSB-EL019114MO

Alternative Names

PYD and CARD domain containing, ASC, CARD5, MGC10332, TMS, TMS-1, TMS1, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain protein 5, target of methylation-induced silencing-1

Reactivity

Mouse

Detection Range

7.81 pg/mL-500 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of pig IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-109
EDTA plasma (n=4)	97	92-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	81-103
1:2	Average %	95
	Range %	90-105
1:4	Average %	95
	Range %	90-108
1:8	Average %	96
	Range %	82-112

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.178	2.152	2.165	2.015
200	1.584	1.560	1.572	1.422
100	0.981	0.950	0.966	0.816
50	0.568	0.564	0.566	0.416
25	0.372	0.361	0.367	0.217
12.5	0.296	0.302	0.299	0.149
6.25	0.252	0.248	0.250	0.100
0	0.152	0.148	0.150	?

Species

Mus musculus

Sensitivity

1.95 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9EPB4
Antigen	PYD and CARD domain containing

Applications

ELISA

Research Area

PYCARD

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505196	CSB-EL019114MO	96T	$695	Add to Cart
BHE10505196	CSB-EL019114MO	5x96T	$3336	Add to Cart
BHE10505196	CSB-EL019114MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.