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Home / ELISA Kit

Mouse Apolipoprotein A-IV(APOA4) ELISA kit

SKU : BHE10505182

This Human PLA2G5 ELISA Kit was designed for the quantitative measurement of Human PLA2G5 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.18 ng/mL-12 ng/mL and the sensitivity is 0.04 ng/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-EL001916MO

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Recovery

The recovery of human PLA2G5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		84		80-88
EDTA plasma (n=4)		96		91-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PLA2G5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		89
		Range %		84-93
1:10		Average %		98
		Range %		84-102
1:20		Average %		104
		Range %		100-110
1:40		Average %		95
		Range %		90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
12	2.207	2.150	2.179	2.023
6	1.768	1.801	1.785	1.629
3	1.202	1.184	1.193	1.037
1.5	0.683	0.634	0.659	0.503
0.75	0.403	0.428	0.416	0.260
0.375	0.321	0.301	0.311	0.155
0.18	0.245	0.227	0.236	0.080
0	0.159	0.152	0.156

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

UniProt ID	P06728

Applications	ELISA
Research Area	APOA4

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10505182	CSB-EL001916MO	96T	$695		Add to Cart
BHE10505182	CSB-EL001916MO	5x96T	$3336		Add to Cart
BHE10505182	CSB-EL001916MO	10x96T	$6602.50		Add to Cart

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