Matrices listed below were spiked with certain level of APO-F and the recovery rates were calculated by comparing the measured value to the expected amount of APO-F in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
92
EDTA plasma(n=5)
92-105
97
heparin plasma(n=5)
87-97
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of APO-F and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
APO-F ELISA Kit allows for the in vitro quantitative determination of APO-F concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse APO-F(Apolipoprotein F) ELISA Kit