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Home / ELISA Kit

Mouse Apelin ELISA kit

SKU : BHE10505177

This Human FDP ELISA Kit was designed for the quantitative measurement of Human FDP protein in serum, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL .

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-E12027m

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

apelin, XNPEP2, apelin, AGTRL1 ligand, peptide ligand for APJ receptor

Recovery

The recovery of human FDP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		106		101-113

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FDP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?		Sample		Serum(n=4)
1:1		Average %		99
		Range %		94-107
1:2		Average %		91
		Range %		87-96
1:4		Average %		95
		Range %		90-100
1:8		Average %		84
		Range %		80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
400	2.615	2.581	2.598	2.396
200	2.150	2.061	2.106	1.904
100	1.588	1.501	1.545	1.343
50	1.042	1.023	1.033	0.831
25	0.697	0.705	0.701	0.499
12.5	0.482	0.523	0.503	0.301
6.25	0.304	0.311	0.308	0.106
0	0.200	0.204	0.202	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Protocol

UniProt ID	Q9R0R4

Applications	ELISA
Research Area	APLN

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10505177	CSB-E12027m	96T	$790		Add to Cart
BHE10505177	CSB-E12027m	5x96T	$3792		Add to Cart
BHE10505177	CSB-E12027m	10x96T	$7505		Add to Cart

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