Mouse ANTXR2 ELISA Kit PicoKine®

Mfr.No.

EK2122

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.015	0.076	0.134	0.239	0.509	0.904	1.457	2.265

Description

Mouse ANTXR2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse ANTXR2 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Anthrax toxin receptor 2; Antxr2

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q32-G318

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse ANTXR2

Subcellular Localization

Membrane. Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	242	1644	5631	217	1771	5410
Standard deviation	14.52	118.36	360.38	14.53	146.99	394.93
CV (%)	6%	7.2%	6.4%	6.7%	8.3%	7.3%

Protein Function

Necessary for cellular interactions with laminin and the extracellular matrix.

Background

Anthrax toxin receptor 2 is a protein that in humans is encoded by the ANTXR2 gene. It is mapped to 5; 5 E3. This gene encodes a receptor for anthrax toxin. The protein binds to collagen IV and laminin, suggesting that it may be involved in extracellular matrix adhesion. Mutations in this gene cause juvenile hyaline fibromatosis and infantile systemic hyalinosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	242	247	246	242	244	2.27	0.9%
Sample 2	1644	1884	1788	1601	1729	113.04	6.5%
Sample 3	5631	5520	5145	4973	5317	268.19	5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	71914
Gene Name	ANTXR2
UniProt ID	Q6DFX2
Protein Name	Anthrax toxin receptor 2

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001660	EK2122	96 wells/kit	$750		Add to Cart

Mouse ANTXR2 ELISA Kit PicoKine®

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