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Home / ELISA Kit

Mouse Antigen peptide transporter 2 (TAP2) ELISA Kit

SKU : BHE13705501

The membrane-associated protein encoded by Tap2 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by Tap2 is involved in antigen presentation. This protein forms a heterodimer with Tap1 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Mutations in the human gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease.

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Mfr.No.	KTE70341
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Antigen peptide transporter 2 (TAP2) ELISA Kit employs a two-site sandwich ELISA to quantitate TAP2.
Alternative Names	TAP2; DAAP-57C1.2; ABC18; ABCB3; APT2; D6S217E; PSF2; RING11; ABC transporter; MHC 2; ATP-binding cassette; sub-family B (MDR/TAP); member 3; antigen peptide transporter 2; peptide supply factor 2; peptide tr
Detection Method	Colorimetric
Kit Components	Mouse Antigen peptide transporter 2 microplate Mouse Antigen peptide transporter 2 standard Mouse Antigen peptide transporter 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Antigen peptide transporter 2 (TAP2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TAP2. No significant cross-reactivity or interference between Mouse TAP2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The membrane-associated protein encoded by Tap2 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by Tap2 is involved in antigen presentation. This protein forms a heterodimer with Tap1 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Mutations in the human gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease.
Notes	This Mouse Antigen peptide transporter 2 (TAP2) ELISA Kit employs a two-site sandwich ELISA to quantitate TAP2 in samples. An antibody specific for TAP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	21355

Applications	ELISA
Applications Notes	This Mouse Antigen peptide transporter 2 (TAP2) ELISA Kit employs a two-site sandwich ELISA to quantitate TAP2 in samples. An antibody specific for TAP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705501	KTE70341	48 T	$339		Add to Cart
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