Mouse anti-nuclear Antibody (IgG) ELISA Kit

Mfr.No.

CSB-E12912m

Sensitivity

1.95 pg/mL

Detection Range

7.8 pg/mL-500 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Capture ELISA

Alternative Names

anti-nuclear Antibody ( IgG)

Recovery

The recovery of human GSTO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-96
EDTA plasma (n=4)	89	85-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GSTO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-94
1:2	Average %	98
	Range %	94-102
1:4	Average %	93
	Range %	86-97
1:8	Average %	104
	Range %	97-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	IgG

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Prednisone treatment inhibits the differentiation of B lymphocytes into plasma cells in MRL/MpSlac-lpr mice. Yan SX.et al,Acta Pharmacol Sin.,2016 36(11):1367-76. Basophil Activation-Dependent Autoantibody and Interleukin-17 Production Exacerbate Systemic Lupus Erythematosus Pan Q.et al,Front Immunol.,Mar 27;8:348. RNAi Silencing of HIF-1α Ameliorates Lupus Development in MRL/lpr Mice Wei Zhao.et al,Inflammation,July 24

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