Matrices listed below were spiked with certain level of Anti-MPV and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-MPV in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-104
97
EDTA plasma(n=5)
89-102
97
heparin plasma(n=5)
90-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-MPV and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-103%
92-102%
90-100%
EDTA plasma(n=5)
92-99%
93-97%
89-96%
heparin plasma(n=5)
94-98%
98-99%
94-98%
Sensitivity
< 1.875ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
Anti-MPV IgG ELISA Kit allows for the in vitro quantitative determination of Anti-MPV concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Anti-MPV IgG (Anti-Monkeypox Virus IgG)ELISA Kit