Mouse anti-double stranded DNA antibody(IgM)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E12911m

Sensitivity

0.39 ng/mL

Detection Range

1.56 ng/mL-100 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Capture ELISA

Alternative Names

anti-double stranded DNA antibody(IgM)

Recovery

The recovery of mouse SP-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-95
EDTA plasma (n=4)	93	90-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	84
	Range %	80-92
1:2000	Average %	96
	Range %	91-105
1:4000	Average %	98
	Range %	93-103
1:8000	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	IgM

Storage	Store at 2-8°C. Please refer to protocol.

Reference	RNAi Silencing of HIF-1α Ameliorates Lupus Development in MRL/lpr Mice Wei Zhao.et al,Inflammation,July 24

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