Matrices listed below were spiked with certain level of anti- Abeta42 anbibody and the recovery rates were calculated by comparing the measured value to the expected amount of anti- Abeta42 anbibody in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-97
94
EDTA plasma(n=5)
88-100
93
heparin plasma(n=5)
81-102
90
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of anti- Abeta42 anbibody and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
80-102%
82-103%
84-103%
EDTA plasma(n=5)
93-101%
91-101%
92-96%
heparin plasma(n=5)
90-98%
94-99%
90-98%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
anti- Abeta42 anbibody ELISA Kit allows for the in vitro quantitative determination of anti- Abeta42 anbibody concentrations in serum, plasma, tissue homogenates and other biological fluids.