Mouse Annexin A1(ANXA1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL001836MO

Reactivity

Mouse

Detection Range

1.37ng/ml - 1000ng/ml

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human OFQ/N spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	80-110
EDTA plasma (n=4)	95	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human OFQ/N in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	80-105
1:2	Average %	96
	Range %	85-114
1:4	Average %	93
	Range %	92-114
1:8	Average %	95
	Range %	80-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

1.37 ng/ml

Limit Of Detection

450 nm

UniProt ID	P10107
Antigen	Annexin A1

Applications

ELISA

Research Area

ANXA1

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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