Matrices listed below were spiked with certain level of Angptl7 and the recovery rates were calculated by comparing the measured value to the expected amount of Angptl7 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
95-103
99
EDTA plasma(n=5)
86-104
95
heparin plasma(n=5)
95-103
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angptl7 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-102%
85-102%
87-98%
EDTA plasma(n=5)
89-96%
82-97%
88-92%
heparin plasma(n=5)
80-98%
80-95%
86-94%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q8R1Q3
Applications
ELISA
Applications Notes
Angptl7 ELISA Kit allows for the in vitro quantitative determination of Angptl7 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Angptl7 (Angiopoietin-related protein 7) ELISA Kit