Mouse angiotensin II(ANG-II) ELISA Kit

Mfr.No.

CSB-E04495m

Sensitivity

0.11 pg/mL

Detection Range

0.45 pg/mL-30 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

angiotensin, angiotensin II, angiotensin 2, ANG2

Recovery

The recovery of rat FXa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	89	85-93

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat FXa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	84
	Range %	80-88
1:200	Average %	102
	Range %	98-109
1:400	Average %	90
	Range %	86-94
1:800	Average %	97
	Range %	92-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	ANG-II

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Control of Adipogenesis by the Autocrine Interplays between Angiotensin (1-7)/Mas receptor and Angiotensin II/AT1 receptor Signaling Pathways Than A et al,J Biol Chem,2013 Pitavastatin-attenuated cardiac dysfunction in mice with dilated cardiomyopathy via regulation of myocardial calcium handling proteins WEI HU et al,Acta pharmaceutica,2014 Mar;64(1):105-15 The effects of different angiotensin II type 1 receptor blockers on the regulation of the ACE-AngII-AT1 and ACE2-Ang(1-7)-Mas axes in pressure overload-induced cardiac remodeling in male mice. Wang X.et al,J Mol Cell Cardiol.,97:180-190. The combination effects of Shen-Ling-Bai-Zhu on promoting apoptosis of transplanted H22 hepatocellular carcinoma in mice receiving chemotherapy. Xi S.et al,J Ethnopharmacol.,190:1-12. Differential cardiac hypertrophy and signaling pathways in pressure versus volume overload. Onitsuka K.et al,J Mol Cell Cardiol,Dec 1

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