Mouse Angiogenin - ANG ELISA Kit

Mfr.No.

E1837Mo

Sensitivity

9.78ng/L

Detection Range

20-6000ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ANG;Angiogenin;Angiogenin-1;Ribonuclease 5;RNase 5

Molecular Weight

16228

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1837Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2170.2	122.05	5.6
Intra-Assay	2	18	1030.3	37.92	3.7
Intra-Assay	3	18	663.1	27.78	4.2

Background

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo (By similarity).

Protocol

These standard curves of E1837Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.148	2.064	2.032
	1.98
1600ng/L	1.437	1.449	1.417
	1.461
800ng/L	0.591	0.574	0.542
	0.557
400ng/L	0.413	0.417	0.384
	0.42
200ng/L	0.206	0.165	0.132
	0.123
0ng/L	0.034	0.032	0
	0.031

Gene ID	11727
UniProt ID	P21570

Research Area	Signal Transduction; Epigenetics and Nuclear Signaling; Neuroscience; Cardiovascular; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109649	E1837Mo	48T	$320		Add to Cart
BHE12109649	E1837Mo	96T	$458		Add to Cart

Mouse Angiogenin - ANG ELISA Kit

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