Mouse Amyloid Beta Peptide 1-42,AB1-42 ELISA Kit

Mfr.No.

E0168Mo

Description

This sandwich kit is for the accurate quantitative detection of amyloid beta peptide 1-42 (also known as Aβ1-42) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein

Reactivity

Mouse

Detection Range

10-2000ng/L

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

4514.1

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

4.26ng/L

Background

Amyloid Beta (Abeta) peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by beta-secretases and gamma-secretases generating diverse Abeta species. Abeta can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons. Alzheimer's disease is characterized by the accumulation of aggregated Abeta peptides in senile plaques and vascular deposits.

UniProt ID	E2GLY0
Antigen	AB1-42

Research Area

Neuroscience

Protocol

These standard curves of E0168Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.345	2.274	2.212
	2.203
600ng/L	1.74	1.766	1.704
	1.791
300ng/L	1.254	1.204	1.142
	1.154
150ng/L	0.66	0.689	0.627
	0.719
75ng/L	0.435	0.44	0.378
	0.446
0ng/L	0.058	0.062	0
	0.066

Precision

We measured random samples of E0168Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	652.6	42.23	6.5
Intra-Assay	2	18	211.5	14.29	6.8
Intra-Assay	3	18	99.5	5.91	5.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12108087	E0168Mo	48T	$320		Add to Cart
BHE12108087	E0168Mo	96T	$458		Add to Cart

Mouse Amyloid Beta Peptide 1-42,AB1-42 ELISA Kit

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