Mouse Aminopeptidase N(ANPEP) ELISA kit

Mfr.No.

CSB-EL001827MO

Sensitivity

9.7 mU/mL

Reactivity

Mouse

Sample Type

Serum, Tissue homogenates, Plasma

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

alanyl (membrane) aminopeptidase, APN, CD13, GP150, LAP1, P150, PEPN, aminopeptidase M, aminopeptidase N, membrane alanine aminopeptidase, microsomal aminopeptidase, myeloid plasma membrane glycoprotein CD13

Recovery

The recovery of human TrpV4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-103
EDTA plasma (n=4)	94	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TrpV4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	101
	Range %	95-108
1:2	Average %	85
	Range %	80-93
1:4	Average %	92
	Range %	88-97
1:8	Average %	90
	Range %	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.004	1.925	1.965	1.840
500	1.682	1.601	1.642	1.517
250	1.350	1.410	1.380	1.255
125	1.023	1.052	1.038	0.913
62.5	0.671	0.703	0.687	0.562
31.25	0.487	0.456	0.472	0.347
15.6	0.272	0.264	0.268	0.143
0	0.128	0.121	0.125	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P97449
Antigen	alanyl (membrane) aminopeptidase

Applications	ELISA
Research Area	ANPEP

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505119	CSB-EL001827MO	96T	$595	Add to Cart
BHE10505119	CSB-EL001827MO	5x96T	$2856	Add to Cart
BHE10505119	CSB-EL001827MO	10x96T	$5652.50	Add to Cart

Mouse Aminopeptidase N(ANPEP) ELISA kit

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