Matrices listed below were spiked with certain level of ALT and the recovery rates were calculated by comparing the measured value to the expected amount of ALT in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-102
96
EDTA plasma(n=5)
88-99
92
heparin plasma(n=5)
85-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
ALT ELISA Kit allows for the in vitro quantitative determination of ALT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse ALT(Alanine Transaminase) ELISA Kit