Mouse alpha-Melanocyte Stimulating Hormone(alpha-MSH) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E15876m

Sensitivity

3.12 pg/mL

Detection Range

12.5 pg/mL-800 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

alpha-Melanocyte Stimulating Hormone(alpha-MSH), alpha-melanocyte stimulating hormone, alpha-MSH

Recovery

The recovery of human CD63 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	98-107
EDTA plasma (n=4)	93	89-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CD63 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-104
1:2	Average %	87
	Range %	81-94
1:4	Average %	98
	Range %	92-106
1:8	Average %	96
	Range %	92-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	alpha-MSH

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Mechanistic Studies on the Use of Polygonum multiflorum for the Treatment of Hair Graying Han MN. et al,Biomed Res Int,2015

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