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Home / ELISA Kit

Mouse Alpha-fetoprotein,AFP ELISA Kit

SKU : BHE10505112

This Rabbit BGLAP ELISA Kit was designed for the quantitative measurement of Rabbit BGLAP protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.31 ng/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-E08282m

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Recovery

The recovery of rabbit OT/BGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		92		90-94
EDTA plasma (n=4)		92		87-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit OT/BGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		87
		Range %		82-93
1:2		Average %		95
		Range %		89-99
1:4		Average %		94
		Range %		90-90
1:8		Average %		94
		Range %		86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
80	2.780	2.701	2.741	2.641
40	2.124	2.056	2.090	1.990
20	1.266	1.234	1.250	1.150
10	0.667	0.621	0.644	0.544
5	0.353	0.335	0.344	0.244
2.5	0.262	0.249	0.256	0.156
1.25	0.219	0.208	0.214	0.114
0	0.102	0.098	0.100	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

UniProt ID	P02772

Applications	ELISA
Research Area	AFP

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10505112	CSB-E08282m	96T	$835		Add to Cart
BHE10505112	CSB-E08282m	5x96T	$4008		Add to Cart
BHE10505112	CSB-E08282m	10x96T	$7932.50		Add to Cart

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