Mouse ALK-1/ACVRL1 ELISA Kit PicoKine®

Mfr.No.

EK1519

Sensitivity

<15 pg/ml

Detection Range

46.9 pg/ml - 3,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	46.9	93.8	187.5	375	750	1500	3000
O.D.	0.032	0.060	0.138	0.239	0.538	1.045	1.895	2.280

Description

Mouse ALK-1/ACVRL1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Acvrl1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Serine/threonine-protein kinase receptor R3;SKR3;2.7.11.30;Activin receptor-like kinase 1;ALK-1;TGF-B superfamily receptor type I;TSR-I;Acvrl1;Acvrlk1, Alk-1;

Molecular Weight

56519 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: D23-P119

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse ALK-1

Subcellular Localization

Cell membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	92	338	1289	84	319	1165
Standard deviation	6.8	23.66	101.83	6.63	27.43	93.2
CV (%)	7.4%	7%	7.9%	7.9%	8.6%	8%

Protein Function

Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well. .

Background

Serine/threonine-protein kinase receptor R3 is an enzyme that in humans is encoded by the ACVRL1 gene. It is also known as activin receptor-like kinase 1, or ALK1. This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	92	92	96	82	90	5.17	5.7%
Sample 2	338	311	358	359	341	19.5	5.7%
Sample 3	1289	1458	1378	1338	1365	61.88	4.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	11482
Gene Name	Acvrl1
UniProt ID	Q61288
Protein Name	Serine/threonine-protein kinase receptor R3

Applications	ELISA
Research Area	Amino Acid Metabolism,Amino Acids,Angiogenesis,Cancer,Cardiovascular,Growth Factors,Growth Factors/Hormones,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction,Vasculature

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001104	EK1519	96 wells/kit	$499		Add to Cart

Mouse ALK-1/ACVRL1 ELISA Kit PicoKine®

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