Matrices listed below were spiked with certain level of Alcam and the recovery rates were calculated by comparing the measured value to the expected amount of Alcam in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-102
96
EDTA plasma(n=5)
86-105
97
heparin plasma(n=5)
87-100
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alcam and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Alcam ELISA Kit allows for the in vitro quantitative determination of Alcam concentrations in serum, plasma, tissue homogenates and other biological fluids.