Matrices listed below were spiked with certain level of ALB and the recovery rates were calculated by comparing the measured value to the expected amount of ALB in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
97
EDTA plasma(n=5)
90-99
95
heparin plasma(n=5)
91-96
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-94%
86-105%
96-104%
EDTA plasma(n=5)
82-100%
83-98%
87-100%
heparin plasma(n=5)
87-98%
82-95%
80-97%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
Applications
ELISA
Applications Notes
ALB ELISA Kit allows for the in vitro quantitative determination of ALB concentrations in serum, plasma, tissue homogenates and other biological fluids.